Product Description

NSF Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70343-50, BS70343-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P46459

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebrafish

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200

Background: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 80KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using NSF antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of BALB-3T3 cells using NSF Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using NSF Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug NSF antibody. Western blot was performed from the immunoprecipitate using NSF antibody at a dilution of 1:3000.

Alternative Name: NSF; SEC18; SKD2

Immunogen: Recombinant fusion protein of human NSF(NP_006169.2).

Conjugate: Unconjugated

Modification: Unmodified