Product Description

Ob-R Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4012-50, ES4012-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: LEPR; DB; OBR; Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from Leptin receptor at AA range: 791-840

Isotype: IgG

Target: Ob-R,

Specificity: Ob-R Polyclonal Antibody detects endogenous levels of Ob-R protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa (PubMed:19159218). Isoform B and isoform A are expressed by NK cells (at protein level) (PubMed:12504075).

Observed Band (KD): 132kD

Fields: Cytokine-cytokine receptor interaction, Neuroactive ligand-receptor interaction, AMPK signaling pathway, JAK-STAT signaling pathway, Adipocytokine signaling pathway, Non-alcoholic fatty liver disease

Function: Domain: The box 1 motif is required for JAK interaction and/or activation. Domain: The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs. Domain: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway. PTM: On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similar

Subcellular Location: Cell membrane, Single-pass type I membrane protein Basolateral cell membrane, [Isoform E]: Secreted

Storage and Stability: -20°C/1 year

Gene Name: LEPR, OBR

Protein Name: Leptin receptor

Human Gene ID: 3953

Human SwissProt Number: P48357

Mouse Gene ID: 16847

Mouse SwissProt Number: P48356

Rat Gene ID: 24536

Rat SwissProt Number: Q62959

Research Use Only