Product Description

Osteocalcin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7961-50, BS7961-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02818

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB: 1:500~1:2000
IF/ICC: 1:50~1:200

Background: This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Osteocalcin polyclonal antibody detects endogenous levels of Osteocalcin protein.

Bioworld Molecular Weight: ~ 12 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of U-87MG cells, using Osteocalcin antibody at 1:1000 dilution., Western blot analysis of extracts of Rat lung, using Osteocalcin antibody at 1:1000 dilution., Immunofluorescence analysis of MG-63 cells using Osteocalcin pAb at dilution of 1:200 (40x lens)., Immunofluorescence analysis of NIH/3T3 cells using Osteocalcin pAb at dilution of 1:200 (40x lens).

Alternative Name: BGLAP; BGP; OC ; OCN; osteocalcin

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Osteocalcin (BGLAP) (NP_954642.1).

Conjugate: Unconjugated

Modification: Unmodified