Product Description

p27 Kip1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67448-50, BS67448-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregμlated in quiescent cells and in cells treated with cAMP or other negative cell cycle regμlators. Downregμlation of p27 can be induced by treatment with interleukin-2 or other mitogens, this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway.

Category: Primary Antibody

Reactivity: Human, Rat, Dog

Host: Rabbit

Applications: WB, IHC

Alternate Names: KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of p27 Kip1 protein.

Molecular Weight: ~ 27 kDa

Pathway: ErbB HER Signaling, Cell Cycle Control G1 S Checkpoin, MAPK Erk in Growth and Differentiation Pathway(, PI3K AKT signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P46527

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only