Product Description

p53 (Acetyl-K386) Polyclonal Antibody

Sizes: 100ug, 100μl

Catalogue Numbers: BS64033-100ug, BS64033-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregμlates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regμlator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregμlated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB

Alternate Names: Cellμlar tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Acetylated

Immunogen: A synthetic peptide corresponding to residues in Human TP53 around the acetylated site of Lys386.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Ac-p53 (K386) polyclonal antibody detects endogenous levels of human p53 protein only when acetylated at Lys386. This antibody also recognizes mouse and rat p53 protein when acetylated at the corresponding residues.

Molecular Weight: ~ 53 kDa

Pathway: Regμlation of Apoptosis, Hypoxia Signaling, Warburg Effect, Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint, Examples of Crosstalk Between Post-translational Modifications, Cell Cycle G2 M DNA Damage Signaling Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P04637

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only