PALLD Polyclonal Antibody-BS74832

PALLD Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74832-50, BS74832-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8WX93

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, , IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IP,50 - 1:200 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 150kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PALLD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded rat ovary using PALLD Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human colon carcinoma using PALLD Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of HeLa cells using PALLD Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PALLD; CGI-151; CGI151; MYN; PNCA1; SIH002; palladin

Immunogen: Recombinant fusion protein of human PALLD(NP_001159582.1).

Conjugate: Unconjugated

Modification: Unmodified