Product Description

PCIF1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78221-50, BS78221-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H4Z3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100 | IP,1:50 - 1:200

Background: Cap-specific adenosine methyltransferase that catalyzes formation of N(6,2'-O-dimethyladenosine cap (m6A(m by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII via interaction with POLR2A and mediates formation of m6A(m co-transcriptionally.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PCIF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunohistochemistry of paraffin-embedded rat brain using PCIF1 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spinal cord using PCIF1 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug PCIF1 antibody. Western blot was performed from the immunoprecipitate using PCIF1 antibody at a dilution of 1:1000.

Alternative Name: PCIF1; C20orf67; PPP1R121; PDX1 C-terminal inhibiting factor 1

Immunogen: Recombinant fusion protein of human PCIF1(NP_071387.1).

Conjugate: Unconjugated

Modification: Unmodified