Product Description

PCNA Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5842-50, BS5842-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granμlar distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, ICC

Alternate Names: Proliferating cell nuclear antigen, Cyclin

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 98-143 of Human PCNA.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PCNA polyclonal antibody detects endogenous levels of PCNA protein.

Molecular Weight: ~ 29, 36 kDa

Pathway: Neuronal and Glial Cell Marker Atlas, DNA Methylation,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P12004

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only