Product Description

PDH-E1 Alpha Monoclonal Antibody

Sizes: 50μL, 100μL

Catalogue Number: BT-MCA1012-50, BT-MCA1012-100

Citations, Manuals and MSDS Available upon request.

Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Research Areas: Signal transduction; Cancer; Metabolism

Target Protein: PDHA1

Applications: WB, IF, ICC

Reactivity: Human,Mouse,Rat,Bovine,Dog,Pig

Clonality: Monoclonal

Host: Mouse

Isotype: IgG

Full Product Name: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

UniProt Accession: Human: P08559; Mouse: P35486; Rat: P26284

Synonyms: PDHA1; PHE1A; Pyruvate dehydrogenase E1 component subunit alpha; somatic form; mitochondrial; PDHE1-A type I

GeneID: Human: 5160; Mouse: 18597

Research Use Only