Product Description

PDK2 (R291) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3913-50, BS3913-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15119

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB 1:500 - 1:1000
IHC 1:50 - 1:100
IP 1:10 - 1:100

Background: The core of PDC is composed of sixty dihydrolypoyl acetyltransferase (E2) subunits that bind directly to PDK2 and enhance PDK2 kinase activity. Upregulation of PDK isoenzymes occurs during starvation conditions, rerouting acetyl-CoA generation by facilitating fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases, in that both require histidine residues for activity. In mammals, transcripts for PDK2 are ubiquitously expressed with high levels in heart and skeletal muscle and decreased levels in spleen and lung.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PDK2 (R291) polyclonal antibody detects endogenous levels of PDK2 protein.

Bioworld Molecular Weight: ~ 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PDK2 (R291) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: L02 whole cell lysate (40ug)
Lane 3: The brain tissue lysate of Mouse (40ug)
Lane 4: The brain tissue lysate of Rat (40ug)
Lane 5: The liver tissue lysate of Mouse (40ug)
Lane 6: The liver tissue lysate of Rat (40ug), Immunoprecipitation of the Brain tissue lysate of Rat using PDK2 (R291) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using PDK2 (R291) pAb #BS3628. “↑”(supernatant); “↓”(deposition)

Alternative Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; PDHK2; PDK2; PDK2; Pyruvate dehydrogenase kinase isoform 2; Pyruvate dehydrogenase kinase; isozyme 2; Pyruvate dehydrogenase lipoamide kinase isozyme 2; mitochondrial

Immunogen: Synthetic peptide, corresponding to amino acids 260-322 of Human PDK2.

Conjugate: Unconjugated

Modification: Unmodified