Product Description

PFKFB1 (E442) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2259-50, BS2259-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16118

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PFKFB1 (E442) polyclonal antibody detects endogenous levels of PFKFB1 protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PFKFB1 (E442) polyclonal antibody in extracts from hela cells., Immunohistochemistry (IHC) analyzes of PFKFB1 (E442) pAb in paraffin-embedded human liver carcinoma tissue at 1:100.

Alternative Name: PFK-2 liver; PFKFB1; PFRX; F6PK; 6PF-2-K/Fru-2; 6-P2ASE liver isozyme; 6-phosphofructo-2-kinase/fructose-2; 6-biphosphatase 1;

Immunogen: Synthetic peptide, corresponding to amino acids 410-460 of Human PFKFB1.

Conjugate: Unconjugated

Modification: Unmodified