PGE1 (Prostaglandin E1) ELISA Kit - ELK9815

PGE1 (Prostaglandin E1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9815-48, ELK9815-96, ELK9815-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Prostaglandin E1; PGE1

Assay Type: Competitive Inhibition

Sensitivity: 9.56 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine

Assay Length: 2h

Research Areas: Metabolic pathway; Infection immunity; Endocrinology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only