Product Description

PGR (phospho-S400)  Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS64224-50, BS64224-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Human progesterone receptor (PR) is expressed as two forms: the fμll length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B. Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription. The activity of PR is regμlated by phosphorylation, at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to fμll length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms. Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2. Mutation of Ser190 resμlts in decreased activity of PR, suggesting that the phosphorylation at Ser190 may be critical to its biological function.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB

Alternate Names: Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human PGR around the phosphorylation site of Serine 400.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PGR(Phospho-S400) polyclonal antibody detects endogenous levels of PGR protein only when phosphorylated at Ser400.

Molecular Weight: ~ 98 kDa

Pathway: Nuclear Receptor Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P06401

Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Research Use Only