Phospho-α-Synuclein-Y133 Polyclonal Antibody-BS79476

Phospho-α-Synuclein-Y133 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79476-50, BS79476-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P37840

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-α-Synuclein-Y133 pAb at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Pervanadate at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Bioworld Molecular Weight: 18KDa

Specificity: Phosphorylated

Alternative Name: PD1; NACP; PARK1; PARK4; SNCA

Immunogen: A synthetic phosphorylated peptide around Y133 of human alpha-Synuclein(NP_000336.1).

Conjugate: Unconjugated

Modification: Phosphorylated