Phospho-AKT1-S473 Polyclonal Antibody-BS79347

Phospho-AKT1-S473 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79347-50, BS79347-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P31749

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IP,1:50 - 1:100

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 62KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of 293F, using Phospho-AKT1-S473 antibody at 1:1570 dilution.293F cells were treated by IGF-1 at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Western blot analysis of NIH/3T3, using Phospho-AKT1-S473 antibody at 1:1570 dilution.NIH/3T3 cells were treated by PDGF at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-AKT1-S473 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-AKT1-S473 pAb. Western blot was performed from the immunoprecipitate using Phospho-AKT1-S473 pAb at a dilution of 1:1000. Jurkat cells were treated by Calyculin A at 37℃ for 30 minutes.

Alternative Name: AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1

Immunogen: A synthetic phosphorylated peptide around S473 of human Akt1(NP_005154.2).

Conjugate: Unconjugated

Modification: Phosphorylated