Phospho-ALOX5-S271 Polyclonal Antibody-BS74009

Phospho-ALOX5-S271 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS74009-50, BS74009-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09917

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HT-29 cells, using Phospho-ALOX5-S271 antibody at 1:1000 dilution. HT-29 cells were treated by PMA/TPA.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Bioworld Molecular Weight: 78kDa

Specificity: Phosphorylated Antibodies

Alternative Name: ALOX5; 5-LO; 5-LOX; 5LPG; LOG5

Immunogen: A synthetic phosphorylated peptide around S271 of human ALOX5(NP_000689.1).

Conjugate: Unconjugated

Modification: Phosphorylated