Product Description

Phospho-AP2M1-T156 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74183-50, BS74183-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96CW1

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T and NIH/3T3 cells, using Phospho-AP2M1-T156 antibody at 1:1000 dilution. 293T cells were treated by Calyculin A for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Hydrogen Peroxide for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.

Bioworld Molecular Weight: 50kDa

Specificity: Phosphorylated

Alternative Name: AP2M1; AP50; CLAPM1; mu2

Immunogen: A synthetic phosphorylated peptide around T156 of human AP2M1(NP_001020376.1).

Conjugate: Unconjugated

Modification: Phosphorylated