Product Description

Phospho-B23 (Thr199) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16486-50, BZ16486-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P06748

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 33 kDa; Observed MW: 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide, using NPM (Phospho-Thr19antibody, Immunofluorescence analysis of Phospho-B23 in HeLa treated with EGF using Phospho-B23 antibody. The picture on the right is blocked with the Phospho- peptide., Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-B23 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right., Western blot analysis of Phospho-B23 in HepG2 lysates using Phospho-B23 antibody. The Lane on the right is blocked with the synthesized peptide.

Alternative Name: B23; NPM

Immunogen: The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220

Conjugate: Unconjugated

Modification: Phosphorylated