Phospho-BCAR1-Y410 Polyclonal Antibody-BS74173

Phospho-BCAR1-Y410 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74173-50, BS74173-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P56945

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-BCAR1-Y410 antibody at 1:5000 dilution. HeLa cells were treated by Pervanadate for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.

Bioworld Molecular Weight: 140kDa

Specificity: Phosphorylated

Alternative Name: BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas

Immunogen: A synthetic phosphorylated peptide around Y410 of human BCAR1(NP_055382.2).

Conjugate: Unconjugated

Modification: Phosphorylated