Phospho-Beclin 1-Ser90 Polyclonal Antibody-BS79510

Phospho-Beclin 1-Ser90 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79510-50, BS79510-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14457

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-Beclin 1-Ser90 antibody at 1:500 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Bioworld Molecular Weight: 60KDa

Specificity: Phosphorylated

Alternative Name: ATG6; VPS30; beclin1; Beclin 1; BECN1; beclin-1

Immunogen: A synthetic phosphorylated peptide around S90 of human Beclin 1(NP_003757.1).

Conjugate: Unconjugated

Modification: Phosphorylated