Phospho-C/EBPB-T235 Polyclonal Antibody-BS79468

Phospho-C/EBPB-T235 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79468-50, BS79468-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17676

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3, using Phospho-C/EBPB-T235 antibody at 1:1000 dilution. NIH/3T3 cells were treated by CIP at 37℃ for 1 hour. NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Bioworld Molecular Weight: 38KDa

Specificity: Phosphorylated

Alternative Name: C/EBP-beta; IL6DBP; NF-IL6; TCF5; CEBP beta; CEBPB

Immunogen: A synthetic phosphorylated peptide around T235 of human C/EBPB(NP_005185.2).

Conjugate: Unconjugated

Modification: Phosphorylated