Product Description

Phospho-cdc25C-S216 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74158-50, BS74158-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P30307

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HT-29 cells, using Phospho-cdc25C-S216 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Bioworld Molecular Weight: 53kDa

Specificity: Phosphorylated

Alternative Name: CDC25C; CDC25; PPP1R60

Immunogen: A synthetic phosphorylated peptide around S216 of human cdc25C(NP_001781.2).

Conjugate: Unconjugated

Modification: Phosphorylated