Phospho-CREB1-S133 Polyclonal Antibody-BS79369

Phospho-CREB1-S133 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79369-50, BS79369-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16220

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:1000 | IHC,1:50 - 1:100

Background: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 40KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CREB1-S133 antibody at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.NIH/3T3 cells were treated by Anisomycin at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded rat brain using Phospho-CREB1-S133 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human tonsil using Phospho-CREB1-S133 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using Phospho-CREB1-S133 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Alternative Name: CREB1; CREB; CREB-1

Immunogen: A synthetic phosphorylated peptide around S133 of human CREB1(NP_604391.1).

Conjugate: Unconjugated

Modification: Phosphorylated