Product Description

phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65784-50, BS65784-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellμlar signal-regμlated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Applications: WB，ICC，

Alternate Names: ERK1 + ERK2 (phospho Thr183/Tyr185), phospho-ERK1/MAPK-1/2(Thr183/Tyr185), ERK 1, ERK 2, ERK-2, ERK1, ERK2, ERT1, ERT2, Extracellμlar signal regμlated kinase 1, Extracellμlar signal regμlated kinase 1, Extracellμlar signal regμlated kinase 2, Extracellμlar signal regμlated kinase 2, Extracellμlar signal-regμlated kinase 2, HS44KDAP, HUMKER1A, Insμlin stimμlated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK1, MAPK2, MGC20180, Microtubμle associated protein 2 kinase, Mitogen activated protein kinase 1, Mitogen activated protein kinase 1, Mitogen activated protein kinase 2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01_MOUSE, p38, p40, p41, p41mapk, p42 MAPK, p42-MAPK, p42MAPK, p42MAPK, p44 ERK1, p44 MAPK, p44ERK1, p44ERK1, p44MAPK, p44MAPK, PRKM 1, PRKM 1, PRKM 2, PRKM 2, PRKM1, PRKM2, Protein kinase mitogen activated 1, Protein kinase mitogen activated 1, Protein kinase mitogen activated 2, Protein kinase mitogen activated 2, Protein tyrosine kinas.聽聽

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse ERK1 around the phosphorylation site of Thr183/Tyr185:FL(p-T)E(p-Y)V

Dilution: WB=1:500-2000，ICC=1:100-500

Purification: affinity purified by Protein A

Specificity: phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody detects endogenous levels of ERK1 + 2 protein only when phosphorylated at Thr183/Tyr185.

Molecular Weight: 42 kD

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P63085

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only