Phospho-Fas-Y291 Polyclonal Antibody-BS6333

Phospho-Fas-Y291 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6333-50, BS6333-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25445

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293 and HeLa cells, using Phospho-Fas-Y291 antibody at 1:1000 dilution. 293 cells were treated by UV for 15-30 minutes. HeLa cells were treated by Doxorubicin for 24 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Bioworld Molecular Weight: 55kDa

Specificity: Phosphorylated Antibodies

Alternative Name: ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6; FAS

Immunogen: A synthetic phosphorylated peptide around Y291 of human FAS(NP_000034.1).

Conjugate: Unconjugated

Modification: Phosphorylated