Product Description

Phospho-FGFR1 (Tyr654) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16414-50, BZ16414-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P11362

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IF: 1/50-1/200 ELISA: 1/10000

Background: Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain. Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues. Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 92 kDa; Observed MW: 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-FGFR1 in Hela lysates using Phospho-FGFR1 antibody., EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide, using FGFR1 antibody., Immunofluorescence analysis of Phospho-FGFR1 in COS7 cells using FGFR1 antibody. The picture on the right is blocked with the Phospho- peptide., Western blot analysis of Phospho-FGFR1 in 293 lysates treated with Insulin using Phospho-FGFR1 antibody. The Lane on the right is blocked with the Phospho- peptide.

Alternative Name: FGFR1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD antigen CD331

Immunogen: The antiserum was produced against synthesized peptide derived from human FGFR1 around the phosphorylation site of Tyr654. AA range:626-675

Conjugate: Unconjugated

Modification: Phosphorylated