Product Description

Phospho-FoxO1-S256 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79355-50, BS79355-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q12778

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:1000 | IF/ICC,1:50 - 1:200

Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 100kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using Phospho-FoxO1-S256 antibody. 293T were treated by CIP at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s., Western blot analysis of extracts of HeLa cells, using Phospho-FoxO1-S256 pAb at 1:1000 dilution or FOXO1 antibody. Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight..
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 2s., Immunofluorescence analysis of NIH/3T3 using Phospho-FoxO1-S256 antibody at dilution of 1:50. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 using Phospho-FoxO1-S256 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: FKH1; FKHR; FOXO1A; FOXO1

Immunogen: A synthetic phosphorylated peptide around S256 of human FoxO1(NP_002006.2).

Conjugate: Unconjugated

Modification: Phosphorylated