Product Description

Phospho-Fyn-S21 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS74003-50, BS74003-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06241

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of A-431 cells, using Phospho-Fyn-S21 antibody at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Bioworld Molecular Weight: 60kDa

Specificity: Phosphorylated Antibodies

Alternative Name: FYN; SLK; SYN; p59-FYN

Immunogen: A synthetic phosphorylated peptide around S21 of human Phospho-Fyn-S21(NP_002028.1).

Conjugate: Unconjugated

Modification: Phosphorylated