Product Description

Phospho-IKK beta (Tyr188) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16477-50, BZ16477-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: O14920

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 87 kDa; Observed MW: 87 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-IKK beta antibody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Sample with blocking peptide on the right., EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide, using IKKbeta (Phospho-Tyr18antibody, Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-IKK beta antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right., Western blot analysis of Phospho-IKK beta in COS7 lysates using Phospho-IKK beta antibody. The Lane on the right is blocked with the synthesized peptide.

Alternative Name: IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB

Immunogen: The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr188. AA range:161-210

Conjugate: Unconjugated

Modification: Phosphorylated