Phospho-IRE1-S724 Polyclonal Antibody-BS79450

Phospho-IRE1-S724 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79450-50, BS79450-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75460

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NIH/3T3, using Phospho-IRE1-S724 antibody at 1:890 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

Bioworld Molecular Weight: 126KDa

Specificity: Phosphorylated

Alternative Name: ERN1; IRE1; IRE1P; IRE1a; hIRE1p

Immunogen: A synthetic phosphorylated peptide around S724 of human IRE1alpha(NP_001424.3?).

Conjugate: Unconjugated

Modification: Phosphorylated