Product Description

Phospho-KLF5 (Ser311) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65572-50, BS65572-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q13887

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

Applications: IHC-P

All Applications: IHC-P=1:100-500（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

Background: Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2;BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-KLF5 (Ser311) polyclonal antibody detects endogenous levels of KLF5 protein only when phosphorylated at Ser311.

Bioworld Molecular Weight: ~50kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5(Ser311)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5(Ser311)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5(Ser311)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF5(Ser311)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

Alternative Name: KLF5 (phospho Ser311); KLF5 (phospho S311); Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5; KLF5_HUMAN.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human KLF5 around the phosphorylation site of Ser311:PG(p-S)PD

Conjugate: Unconjugated

Modification: Phosphorylation