Product Description

Phospho-MEK1-T286 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6391-50, BS6391-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02750

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-MEK1-T286 antibody at 1:2000 dilution or MEK1 antibody. HeLa cells were treated by nocodazole at 37℃ for 20 hours or treated by ATP at 30℃ for 1 hour. NIH/3T3 cells were treated by Nocodazole at 37℃ for 20 hours or treated by ATP at 30℃ for 1 hour. PC-12 cells were treated by Nocodazole at 37℃ for 16 hours or treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.

Bioworld Molecular Weight: 43kDa

Specificity: Phosphorylated Antibodies

Alternative Name: CFC3; MAPKK1; MEK1; MKK1; PRKMK1; MAP2K1

Immunogen: A synthetic phosphorylated peptide around T286 of human MEK1(NP_002746.1).

Conjugate: Unconjugated

Modification: Phosphorylated