Phospho-Na+/K+-ATPase-S16 Polyclonal Antibody-BS79471

Phospho-Na+/K+-ATPase-S16 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79471-50, BS79471-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05023

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using Phospho-Na+/K+-ATPase-S16 antibody at 1:1000 dilution. 293T cells were treated by PMA/TPA at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 112kDa

Specificity: Phosphorylated

Alternative Name: ATP1A1; ATPase Na+/K+ transporting subunit alpha 1; ATP1A; CMT2DD; HOMGSMR2

Immunogen: A synthetic phosphorylated peptide around S16 of human Na+/K+-ATPase(NP_000692.2).

Conjugate: Unconjugated

Modification: Phosphorylated