Product Description

phospho-NeuroD1 (Ser274) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65833-50, BS65833-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q13562

Host: Rabbit

Reactivity: Human, Mouse, Rat, Pig, Cow,Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -6°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6. 0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes;Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuroD1 (Ser274)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Bioworld Molecular Weight: ~40 kDa

Specificity: phospho-NeuroD1 (Ser274)Polyclonal Antibody detects endogenous levels of NeuroD1 protein only when phosphorylated at Y274.

Alternative Name: NeuroD1 (phospho S274); p-NeuroD1 (phospho S274); atonal; basic helix loop helix transcription factor; BETA 2; Beta cell E box transactivator 2; BETA2; BHF 1; BHF1; bHLHa3; class A basic helix loop helix protein 3; Class A basic helix-loop-helix protein 3; MODY 6; MODY6; NDF1_HUMAN; NEUROD; NeuroD1; Neurogenic differentiation 1; Neurogenic differentiation factor 1; neurogenic helix loop helix protein NEUROD; NIDDM

Immunogen: KLH conjugated Synthesised acetylpeptide derived from human NeuroD1 around the acetylation site of Ser274:PL(p-S)PP

Conjugate: Unconjugated

Modification: Phosphorylation