Phospho-NF-kB p65/RelA-S468 Polyclonal Antibody-BS79373

Phospho-NF-kB p65/RelA-S468 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79373-50, BS79373-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q04206

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3 cells, using Phospho-NF-kB p65-S468 pAb at 1:2000 dilution or NF-kB p65 antibody. NIH/3T3 cells were treated by TNF-α at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 60kDa

Specificity: Phosphorylated

Alternative Name: NFKB3; p65; NF-kB p65; RELA; CMCU

Immunogen: A synthetic phosphorylated peptide around S468 of human Phospho-NF-kB p65/RelA-S468(NP_001138610.1).

Conjugate: Unconjugated

Modification: Phosphorylated