Phospho-NF-kB p65/RelA-T434 Polyclonal Antibody-BS79442

Phospho-NF-kB p65/RelA-T434 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79442-50, BS79442-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q04206

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3 cells, using Phospho-NF-kB p65/RelA-T434 pAb at 1:1000 dilution or RelA antibody. NIH/3T3 cells were treated by TNF-α at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5min.

Bioworld Molecular Weight: 58kDa

Specificity: Phosphorylated

Alternative Name: NFKB3; p65; NF-kB p65; RELA; CMCU

Immunogen: A synthetic phosphorylated peptide around T435 of human RelA(NP_068810.3).

Conjugate: Unconjugated

Modification: Phosphorylated