Product Description

phospho-NF1 (Ser2515) Polyclonal Antibody

Size: 100µl

Catalogue Number: BS65916-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P21359

Host: Rabbit

Reactivity: Human, Mouse, Rat, Horse, Rabbit,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Neurofibromin is a product of the tumor suppressor gene, Neurofibromatosis type I. Neurofibromin is known to have GTPase activity that modulates the ras pathway. The absence of or alteration of the neurofibromin protein may lead to Neurofibromatosis disease. This protein has not been purified, therefore, most of the information regarding this protein has been deduced from homology analysis of its gene sequence.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: phospho-NF1 (Ser2515) Polyclonal Antibody detects endogenous levels of NF1 protein only when phosphorylated at Ser2515.

Bioworld Molecular Weight: 147/319kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-NF1(Ser2515)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-NF1(Ser2515)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: NF1(phospho S2515); NF1(phospho Ser2515); p-NF1(S2515); DKFZp686J1293; FLJ21220; Neurofibromatosis Noonan syndrome; Neurofibromatosis related protein NF 1; Neurofibromatosis related protein NF1; neurofibromatosis type I; Neurofibromatosis-related protein NF-1; Neurofibromin 1; Neurofibromin truncated; Neurofibromin1; NF 1; NF; NF1; NF1_HUMAN; NFNS; Type 1 Neurofibromatosis; von Recklinghausen disease neurofibromin; von Recklinghausen disease related protein VRNF; VRNF; WATS; Watson disease related protein WSS; Watson syndrome; WSS.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NF1 around the phosphorylation site of Ser2515:QT(p-S)PR

Conjugate: Unconjugated

Modification: Phosphorylation