Product Description

Phospho-p53 (Ser15) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16379-50, BZ16379-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P04637

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC-F, IHC-P, IP, , ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ELISA: 1/10000

Background: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 44 kDa; Observed MW: 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-p53 in HeLa treated with HU lysates using Phospho-p53 antibody. The Lane on the right is blocked with the Phospho- peptide., Immunohistochemistry analysis of paraffin-embedded rat spleen tissue using Phospho-p53 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of Phospho-p53 in rat spleen tissue using antibody., Western blot analysis of Phospho-p53 in untreated and Phospho-p53 treated with HU in 293T lysates using Phospho-p53 antibody

Alternative Name: TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Immunogen: The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser15. AA range:1-50

Conjugate: Unconjugated

Modification: Phosphorylated