Product Description

Phospho-PKA alpha/beta/gamma (Thr197) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BZ16474-50, BZ16474-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: PRKACA (protein kinase cAMP-activated catalytic subunit alpha) encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regμlatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regμlatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regμlatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellμlar processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing resμlts in mμltiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

Alternate Names: PRKACA, PKACA, cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alpha, PRKACB, cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACG, cAMP-dependent protein kinase catalytic subunit gamma, PKA C-gamma

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: The antiserum was produced against synthesized peptide derived from human PKA CAT around the phosphorylation site of Thr197. AA range:166-215

Dilution: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Purification: Affinity Purified

Specificity: IgG

Molecular Weight: Calcμlated MW: 40 kDa, Observed MW: 40 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17612/P22694/P22612

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only