Phospho-PKC (Pan) (βII Ser660) Polyclonal Antibody-BS74145

Phospho-PKC (Pan) (βII Ser660) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74145-50, BS74145-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17252/P05771/Q05655/Q02156/P24723/Q04759

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using Phospho-PKC pAb at 1:1000 dilution or PRKCQ antibody. 293T cells were treated by PMA/TPA for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 95kDa

Specificity: Phosphorylated

Alternative Name: PRKCA/PRKCB/PRKCD/PRKCE/PRKCH/PRKCQ

Immunogen: A synthetic phosphorylated peptide around S660 of human PKC (pan) βIIPRKCA(NP_002728.2).

Conjugate: Unconjugated

Modification: Phosphorylated