Product Description

Phospho-POLR2A CTD-S7 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79466-50, BS79466-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P24928

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of MCF7 cells, using Phospho-POLR2A CTD-S7 antibody at 1:1000 dilution. MCF7 cells were treated by Doxorubicin at 37℃ for 24 hours. MCF7 cells were treated by nocodazole at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 270kDa

Specificity: Phosphorylated

Alternative Name: POLR2A; POLR2; POLRA; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1; Pol II

Immunogen: A phospho specific peptide corresponding to residues surrounding S7 of human POLR2A CTD repeatYSPTSPS.

Conjugate: Unconjugated

Modification: Phosphorylated