Phospho-PRKCQ-T538 Polyclonal Antibody-BS74176

Phospho-PRKCQ-T538 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74176-50, BS74176-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q04759

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Jurkat and HeLa cells, using Phospho-PRKCQ-T538 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA for 10 minutes. HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.

Bioworld Molecular Weight: 98kDa

Specificity: Phosphorylated

Alternative Name: PRKCQ; PRKCT; nPKC-theta

Immunogen: A synthetic phosphorylated peptide around T538 of human PRKCQ(NP_006248.1).

Conjugate: Unconjugated

Modification: Phosphorylated