Product Description

Phospho-Pyruvate Dehydrogenase E1-alpha subunit (Ser293) Recombinant Rabbit mAb

Sizes: 50μl, 100μl

Catalogue Numbers: BS43266-50, BS43266-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial mμltienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of mμltiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: PDHA, PDHAD, PHE1A, PDHCE1A

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding
Ser293 of human Pyruvate Dehydrogenase E1-alpha subunit

Dilution: WB: 1:1000 IHC: 1:100 IP: 1:20

Purification: Affinity Purification

Specificity: IgG

Molecular Weight: 43 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P08559

Product: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Research Use Only