Product Description

Phospho-Raf1 (Ser259) Recombinant Rabbit mAb

Sizes: 50μl, 100μl

Catalogue Numbers: BS43021-50, BS43021-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is the cellμlar homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellμlar RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jμl 2008]

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: NS5, CRAF, Raf-1, c-Raf, CMD1NN

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding
Ser259 of human Raf1

Dilution: WB: 1:1000 IHC: 1:20

Purification: Affinity Purification

Specificity: IgG

Molecular Weight: 73 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P04049

Product: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Research Use Only