Phospho-RB-S608 Polyclonal Antibody-BS79404

Phospho-RB-S608 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79404-50, BS79404-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06400

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Jurkat cells, using Phospho-RB-S608 antibody at 1:2000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.

Bioworld Molecular Weight: 110kDa

Specificity: Phosphorylated

Alternative Name: OSRC; PPP1R130; RB; p105-Rb; pRb; pp110; RB1; Rb

Immunogen: A synthetic phosphorylated peptide around S608 of human RB(NP_000312.2).

Conjugate: Unconjugated

Modification: Phosphorylated