Phospho-SERCA2/ATP2A2-Thr484 Polyclonal Antibody-BS79473

Phospho-SERCA2/ATP2A2-Thr484 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79473-50, BS79473-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16615

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-SERCA2/ATP2A2-Thr484 antibody at 1:1000 dilution. NIH/3T3 cells were treated by ATP at 30℃ for 1 hour. C6 cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 114KkDa/140kDa

Specificity: Phosphorylated

Alternative Name: ATP2B; DAR; DD; SERCA2; ATP2A2/SERCA2; ATP2A2

Immunogen: A synthetic phosphorylated peptide around T484 of SERCA2/ATP2A2(NP_733765.1).

Conjugate: Unconjugated

Modification: Phosphorylated