Product Description

Phospho-SMC1-S957 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6416-50, BS6416-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14683

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IP,1:50 - 1:100

Background: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated Antibodies

Bioworld Molecular Weight: 160kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-SMC1-S957 antibody at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3s., Western blot analysis of extracts of HeLa cells, using Phospho-SMC1-S957 pAb at 1:1000 dilution or SMC1A antibody. HeLa cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3s., Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-SMC1-S957 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Phospho-SMC1-S957 antibody. Western blot was performed from the immunoprecipitate using Phospho-SMC1-S957 antibody at a dilution of 1:1000.

Alternative Name: SMC1A; CDLS2; DXS423E; SB1.8; SMC1; SMC1L1; SMC1alpha; SMCB

Immunogen: A synthetic phosphorylated peptide around S957 of human SMC1A(NP_006297.2).

Conjugate: Unconjugated

Modification: Phosphorylated