Phospho-STING/TMEM173-S366 Polyclonal Antibody-BS79500

Phospho-STING/TMEM173-S366 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79500-50, BS79500-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q86WV6

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of THP-1, using Phospho-STING/TMEM173-S366 antibody at 1:660 dilution. THP-1 cells were transfection with poly for 3 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 120s.

Bioworld Molecular Weight: 33KDa/40KDa

Specificity: Phosphorylated

Alternative Name: TMEM173; ERIS; MITA; MPYS; NET23; SAVI; STING; hMITA; hSTING

Immunogen: A synthetic phosphorylated peptide around S366 of human STING/TMEM173(NP_938023.1).

Conjugate: Unconjugated

Modification: Phosphorylated