Product Description

Phospho-STK11-S334 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79415-50, BS79415-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15831

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-STK11-S334 antibody at 1:1000 dilution. Hela cells were treated by UV at room temperature for 30 minutes and PMA at 37℃ for 30 minutes after serum-starvation
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Bioworld Molecular Weight: 54KDa

Specificity: Phosphorylated

Alternative Name: LKB1; PJS; hLKB1; STK11

Immunogen: A synthetic phosphorylated peptide around S334 of human STK11(NP_000446.1).

Conjugate: Unconjugated

Modification: Phosphorylated