Product Description

Phospho-TBC1D4-T642 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74174-50, BS74174-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60343

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using Phospho-TBC1D4-T642 antibody at 1:2000 dilution. 293T cells were treated by IGF-1 for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

Bioworld Molecular Weight: 160kDa

Specificity: Phosphorylated

Alternative Name: AS160; NIDDM5; TBC1D4

Immunogen: A synthetic phosphorylated peptide around T642 of human TBC1D4(NP_055647.2).

Conjugate: Unconjugated

Modification: Phosphorylated